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. 2008 Feb 1;74(7):1988–1996. doi: 10.1128/AEM.01851-07

FIG. 5.

FIG. 5.

Transcriptional analysis of butBA expression. (A) RT-PCR analysis of butBA mRNA levels in Lactococcus bv. diacetylactis strain CRL264 grown in M17G or M17GC medium at pH 5.0 or 7.0. The RT-PCRs were performed as described in Materials and Methods, and the results are depicted as in Fig. 2. (B) Analysis of the initiation of transcription from butBA promoter determined by primer extension in Lactococcus bv. diacetylactis strain CRL264 grown in M17G at pH 7.0 and 5.0. The extension product is indicated by an arrow (+1). (C) Organization of the Lactococcus bv. diacetylactis butBA genes, with a schematic representation of pIL77 and the β-galactosidase activity encoded by IL1403(pIL77) grown in M17G at the indicated pHs. The location and size of the fragment fused to lacLM in pIL77 are also shown. In the sequence of the butBA promoter region, the upper lines indicate the −35 box, the −10 extended box, and the transcriptional start site (+1). The ButB translational start site is underlined with a discontinuous line.