TABLE 2.

Influence of acidic growth in levels of proteins involved in citrate and glucose metabolisms

Protein		Fold inductiona		pI	Mr	Scoreb	Coveragec (%)
Function	Name	CRL30	CRL264
Citrate metabolism
Citrate lyase α subunitd	CitF	1.36	1.45	5.20	55,440	106	12
Citrate lyase β subunit	CitE	1.81	2.06	4.84	33,307	252	55
Oxalacetate decarboxylase	CitM	1.82	2.16	5.21	40,475	137	26
Pyruvate metabolism
α-Acetolactate synthase	Als	1.40	1.24	4.80	60,863	255	34
l-Lactate dehydrogenase	Ldh	0.94	1.06	4.90	35,050	176	25
Pdh E1 component α subunit	PdhA	1.18	1.16	5.10	41,325	99	20
Pdh E1 component β subunit	PdhB	1.04	1.05	4.73	35,210	197	41
Pdh lipoamide dehydrogenase component	PdhD	1.18	1.17	4.85	59,867	149	24
Phosphate acetyltransferase	Pta	0.89	1.07	4.91	35,256	126	28
Glucolysis
Fructose-bisphosphate aldolase	FbaA	0.95	1.01	5.04	31,989	260	41
Phosphoglycerate kinase	Pgk	1.03	1.14	5.00	42,070	222	48
Pyruvate kinase	Pyk	1.19	0.87	5.21	54,255	110	19

^aThe fold induction at acidic pH values were calculated by dividing the mean values of the spot intensities (from three independent experiments) obtained from cultures grown at pH 5.0 or 7.0. The mean values and the standard deviation of the spot intensities are depicted in Table S1 in the supplemental material.

^bThe score is −10·log(P), where P is the probability that the observed match is a random event. Protein scores greater than 76 are significant (P < 0.05).

^cCoverage is the ratio of amino acids (identified in peptides/in theoretical peptides from sequence data) expressed as a percentage.

^dDetected a proteolytic C-terminal fragment of CitF with an M_r of 22,000 and a pI of 4.6.