TABLE 1.
S. cerevisiae strains and plasmids used in this study
| S. cerevisiae strain or plasmid | Relevant genotype or phenotype | Reference |
|---|---|---|
| Strains | ||
| BWY1 | MATaleu2-3,112 ura3-52 trp1-289 his3-Δ1 MAL2-8cSUC; unknown beneficial mutations for l-arabinose utilization | This work |
| BWY1-4M | BWY1 with plasmids YEparaA, YEparaBG361A, YEparaD, and YEpGAL2 | This work |
| BWY1-4S | BWY1 with plasmids YEparaAsynth, YEparaBsynth, YEparaDsynth, and YEpGAL2 | This work |
| Plasmids | ||
| YEparaA | pHXT7-AraA-tCYC1, HIS3 | 5 |
| YEpGAL2 | pHXT7-GAL2-tCYC, URA3 | 5 |
| YEparaB | pHXT7-AraB-tCYC1, TRP1 | 5 |
| YEparaD | pHXT7-AraD-tCYC1, LEU2 | 5 |
| YEparaAsynth | pHXT7-codon-optimized AraAB. liche-tCYC1, HIS3 | This work |
| YEparaBsynth | pHXT7-codon-optimized AraB-tCYC1, TRP1 | This work |
| YEparaDsynth | pHXT7-codon-optimized AraD-tCYC1, LEU2 | This work |
| p423H7 | HIS3 | 13 |
| p424H7 | TRP1 | 13 |
| p425H7 | LEU2 | 13 |
| p426H7 | URA3 | 13 |
| YEparaAB. liche | pHXT7-AraA from B. licheniformis tCYC1, HIS3 | This work |
| YEparaAC. aceto | pHXT7-AraA from C. acetobutylicum tCYC1, HIS3 | This work |
| YEparaAP. pento | pHXT7-AraA from P. pentosaceus tCYC1, HIS3 | This work |
| YEparaAL. planta | pHXT7-AraA from L. plantarum tCYC1, HIS3 | This work |
| YEparaAL. mesent | pHXT7-AraA from L. mesenteroides tCYC1, HIS3 | This work |
| YEparaAB. liche-6HIS | pHXT7-AraA from B. licheniformis tCYC1, HIS3; His6 epitope | This work |
| YEparaAC. aceto-6HIS | pHXT7-AraA from C. acetobutylicum tCYC1, HIS3; His6 epitope | This work |
| YEparaAB. subtilis-6HIS | pHXT7-AraA from B. subtilis tCYC1, HIS3; His6 epitope | This work |