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. 2003 Nov;77(21):11471–11479. doi: 10.1128/JVI.77.21.11471-11479.2003

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Copyright © 2003, American Society for Microbiology

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FIG. 1. — Proliferation of Atlantic salmon PBL stimulated with CpG ODN, non-CpG ODN, calf thymus DNA, and bacterial LPS. Atlantic salmon PBL (8 × 10⁵ cells/well) were used for cell proliferation studies. The cells were cultured with various concentrations of the stimulants for 6 days, and proliferation was assessed by measuring [³H]thymidine incorporation. The data are reported as the mean counts per minute ± standard deviation (SD) of the mean for six fish or as the stimulation index ± SD and are representative of at least two independent experiments. (a) The results are presented in a semilogarithmic scale; the closed symbols represent cultures stimulated with CpG ODN 1681, and the open symbols represent cultures stimulated with control GpC ODN. (b) The stimulation with ODN 1681 and 1668 (at 2 and 8 μM [i.e., 12 and 48 μg ml⁻¹, respectively]) is compared with those with non-CpG, bacterial and calf thymus DNA (at 12.5 and 50 μg ml⁻¹), and LPS (at 10 and 50 μg ml⁻¹). **, statistical significance (P < 0.01) in comparison to the control.

FIG. 1. — Proliferation of Atlantic salmon PBL stimulated with CpG ODN, non-CpG ODN, calf thymus DNA, and bacterial LPS. Atlantic salmon PBL (8 × 10⁵ cells/well) were used for cell proliferation studies. The cells were cultured with various concentrations of the stimulants for 6 days, and proliferation was assessed by measuring [³H]thymidine incorporation. The data are reported as the mean counts per minute ± standard deviation (SD) of the mean for six fish or as the stimulation index ± SD and are representative of at least two independent experiments. (a) The results are presented in a semilogarithmic scale; the closed symbols represent cultures stimulated with CpG ODN 1681, and the open symbols represent cultures stimulated with control GpC ODN. (b) The stimulation with ODN 1681 and 1668 (at 2 and 8 μM [i.e., 12 and 48 μg ml⁻¹, respectively]) is compared with those with non-CpG, bacterial and calf thymus DNA (at 12.5 and 50 μg ml⁻¹), and LPS (at 10 and 50 μg ml⁻¹). **, statistical significance (P < 0.01) in comparison to the control.