FIG. 2.

Screening for the optimal sequence of phosphothiorate ODN to activate Atlantic salmon leukocytes. Leukocytes were incubated with different ODN (2 μM) with the sequences indicated (CG or GC dinucleotides are underlined). (A) Supernatants were harvested from HK leukocytes 48 h poststimulation, and antiviral activity was detected. The results are given as the mean IFN-like activity for pooled supernatants from six different fish and are representative of at least four different experiments. (B) Cell proliferation of Atlantic salmon PBL assessed by thymidine incorporation. The data are reported as the mean counts per minute ± standard deviation of the mean for six fish and are representative of at least two independent experiments.