TABLE 1.
Dose-response studya
| Treatment and dose | Tank | Cumulative mortality (%) | Mean cumulative mortality (%) | RPS | Mean RPS | Statistical significance (χ2 test)b |
|---|---|---|---|---|---|---|
| Saline | A | 75 | 77 | |||
| B | 78 | |||||
| Poly(I-C), 100 μg | A | 43 | 55 | 43 | 28 | |
| B | 66 | 12 | ||||
| CpG | ||||||
| 1 μg | A | 67 | 72 | 11 | 6 | n.s. |
| B | 77 | 1 | ||||
| 10 μg | A | 58 | 69 | 23 | 11 | |
| B | 79 | −1 | ||||
| 50 μg | A | 56 | 61 | 25 | 21 | P < 0.01 |
| B | 65 | 17 | ||||
| 100 μg | A | 55 | 62 | 27 | 20 | P < 0.05 |
| B | 69 | 12 |
a
Cumulative mortality was calculated following IPNV challenge of CpG ODN- and poly(I-C)-injected fish 7 days postinjection. RPS = {1 − [% mortality of fish given CpG ODN or poly(I-C)/% mortality of fish given saline]} × 100.
b
Statistical significance is based on a comparison to results for the saline group. n.s., not significant.