TABLE 2.
Cumulative mortality and viral titers following viral challenge of CpG-, non-CpG-, and poly(I-C)-injected Atlantic salmon on indicated days postinjection
| Treatment | Fish in tank C
|
Fish in tanks A and B
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Day 7
|
Day 21
|
Mean cumulative mortality (%)b
|
Statistical significance (χ2 test)c | ||||||||
| No. of virus negative/total no. | No. of dead/total no. | No. with titer of >102.40/total no.a | No. of virus negative/total no. | No. of dead/total no. | No. with indicated titer/total no.a
|
Day 21 | Day 37 | ||||
| >102.40 | >104.00 | >106.00 | |||||||||
| Saline | 4/5 | 0/5 | 1/5 | 2/5 | 1/5 | 2/5 | 17 | 54 (76/140) | |||
| Non-CpG | 3/5 | 0/5 | 2/5 | 0/5 | 2/5 | 1/5 | 2/5 | 14 | 55 (77/140) | ||
| CpG | 5/5 | 0/5 | 3/5 | 0/5 | 1/5 | 1/5 | 8 | 37 (27/73) | P < 0.05 | ||
| Poly(I-C) | 5/5 | 0/5 | 5/5 | 0/5 | 5 | 41 (57/140) | P < 0.05 | ||||
a
Titer measured in TCID50 per gram of tissue.
b
Mean results (number of dead/total number of fish) for tanks A and B are given except for the results from the CpG-infected group. which are for tank A only.
c
Statistical significance is based on a comparison between results for fish on day 37 postinjection and results for the saline group.