Inheritance and copy number of plasmid pZJY41 in M. xanthus DZ1. (A) Inheritance of plasmid pZJY41 in M. xanthus DZ1 in the absence of antibiotic selection. (B) Genomic DNA, including the chromosome and plasmid, were isolated and then diluted 10-, 100-, and 1,000-fold and electrophoresed in a 0.7% agarose gel at 35 V/37 cm for 12 h. Lambda HindIII DNA was used as the size marker. Chr, chromosome.