TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or descriptiona | Source |
|---|---|---|
| Strains | ||
| M. fulvus 124B02 | Wild-type strain, containing plasmid pMF1 | This study |
| M. xanthus DK1622 | Wild-type strain | D. Kaiser, Stanford University |
| M. xanthus DZ1 | Nonmotile, nonfruiting, dispersed growth | D. R. Zusman, University of California, Berkeley |
| M. xanthus DK10410 | ΔpilA mutant of DK1622 | M. H. Singer, University of California, Davisb |
| E. coli DH5α | supE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Life Technologies Inc. |
| Plasmids | ||
| pSP72 | Ampr | Life Technologies Inc. |
| ColE I | Kmr | D. Kaiser, Stanford University |
| pMF1 | Cryptic plasmid from M. fulvus 124B02 | This study |
| pZJY1 | Ampr Kmr; pSP72 with insertion of 1.2-kb Kmr gene from ColE I | This study |
| pZJY2 | Ampr Kmr; pZJY1 with insertion of 14.1-kb XbaI fragment (bp 1 to 14132) of pMF1 | This study |
| pZJY7 | Ampr Kmr; pZJY1 with insertion of 3.0-kb SalI fragment (bp 10953 to 13980) of pMF1 | This study |
| pZJY8 | Ampr Kmr; pZJY1 with insertion of 940-bp SalI-SacII fragment of pZJY7 (bp 10953 to 11891) of pMF1 | This study |
| pZJY9 | Ampr Kmr; pZJY1 with insertion of 2.4-kb MluI fragment of pZJY7 (bp 11183 to 13608) of pMF1 | This study |
| pZJY13 | Ampr Kmr; pZJY1 with insertion of 1.4-kb fragment (bp 12527 to 13980) of pMF1 | This study |
| pZJY15 | Ampr Kmr; pZJY1 with insertion of 2.2-kb fragment (bp 11645 to 13853) of pMF1 | This study |
| pZJY41 | Stable shuttle plasmid from a DZ1 transformant of pZJY7 | This study |
| pZJY156 | Stable shuttle plasmid from a DK1622 transformant of pZJY15 | This study |
| pZJY24_30 | Ampr Kmr; pZJY41 containing the pilA gene, parallelc | This study |
| pZJY25_35 | Ampr Kmr; pZJY156 containing the pilA gene, parallelc | This study |
a
Ampr, ampicillin resistance; Kmr, kanamycin resistance.
b
See reference 49.
c
The pilA gene is in the same orientation as the pMF1.14 gene of pMF1 in pZJY41 or pZJY156.