TABLE 2.
Stoichiometry of substrate degradation by strain UIT cocultured with M. hungatei (incubated for 100 days)
| Substrate | Initial substrate concn (mM) | Maximum concn of the following transient intermediate detected
|
Final substrate or product concn
|
Degradability in 100 days (%) | Electron recovery (%)b | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Hydrogen (Pa) | Benzoate (mM) | Phenol (mM) | 4-Hydroxybenzoate (mM) | Substrate (mM) | Acetate (mM) | Methanea | ||||
| Isophthalate | 2.17 | 20 | 8 | 0.61 | 4.53 | 1.04 | 72 | 95 | ||
| Phenol | 4.64 | 20 | 11 | 4 | 3.31 | 4.11 | 0.71 | 31 | 104 | |
| 4-Hydroxybenzoate | 3.85 | 20 | 92 | 20 | 0.70 | 8.43 | 1.49 | 82 | 90 | |
a
The methane values are expressed as millimoles of methane formed in 1 liter of culture.
b
The electron balance was calculated based only on the substrate utilized and acetate and methane formed (biomass was excluded).