TABLE 1.

Oligonucleotides used for gene content analysis in non-O157 STEC

Gene targeta	Oligonucleotide sequence (5′-3′)		Product size (bp)	Annealing temp (°C)
	Primer	Primer 2
nleA	ATGAACATTCAACCGACCATAC	GACTCTTGTTTCTTGGATTATATCAAA	1,296	55
nleB	GGAAGTTTGTTTACAGAGACG	AAAATGCCGCTTGATACC	297	55
nleB2	GTTAATACTAAGCAGCATCC	CCATATCAAGATAGATACACC	475	52
nleC	ACAGTCCAACTTCAACTTTTCC	ATCGTACCCAGCCTTTCG	777	55
nleD	GGTATTACATCAGTCATCAAGG	TTGTGGAAAACATGGAGC	426	55
nleE	GTATAACCAGAGGAGTAGC	GATCTTACAACAAATGTCC	260	52
nleF	ATGTTACCAACAAGTGGTTCTTC	ATCCACATTGTAAAGATCCTTTGTT	567	55
nleG (Z6010)	ATGTTATCGCCCTCTTCTATAAAT	ACTTAATACTACACTAATAAGATCCA	902	55
nleG2-1 (Z6025)	ACCAGAAACCTGACTTCG	CAGCATCTTCATATACTACAGC	406	55
nleG2-3 (Z2149)	GGATGGAACCATACCTGG	CGCAATCAATTGCTAATGC	551	56
nleG5-2 (Z2151)	TGGAGGCTTTACGTCATGTCG	CCGGAACAAAGGGTTCACG	504	55
nleG6-2 (Z2150)	CGGGTCAGTGGATGATATGAGC	AAGTAGCATCTAGCGGTCGAGG	424	55
nleG9 (Z2560)	GTTCGTGCCCGAATTGTAGC	CACCAACCAAACGAGAAAATG	409	55
nleH1-2 (Z6021)	AACGCCTTATATTTTACC	AGCACAATTATCTCTTCC	589	52
nleH1-1 (Z0989)	GTTACCACCTTAAGTATCC	GTTTCTCATGAACACTCC	456	55
ent/espL2	GAATAACAATCACTCCTCACC	TTACAGTGCCCGATTACG	233	55

^aDesignations in parentheses are alternate gene names used previously.