TABLE 2.
Specifications of the σmarY1-based T. matsutake geotypes in Asia
| Geotype and subgeotype | Profile shown by analytical system (primer set)/size of PCR-amplified DNA (bp)a
|
Geographical distribution | ||||||
|---|---|---|---|---|---|---|---|---|
| pDGSL313-1/pS48
|
pDGSL719-2A/pS48 (457 bp) | |||||||
| 573 | 493b | 450c | 337b | 299c | 273 | |||
| A | V | + | V | + | V | V | V | Far East |
| A1 | − | + | − | + | − | V | − | Japan |
| A2 | − | + | + | + | + | V | V | Japan |
| A3 | − | + | − | + | − | V | + | Korean Peninsula |
| A4 | + | + | V | + | V | V | + | China (northeastern provinces) |
| B | − | − | − | − | − | + | − | China (southwestern provinces)-Bhutan |
a
DNA profiles predominant in a given geotype or subgeotype are shown (Fig. 3 to 5 show raw data). DNA profiles showing positive and negative signals of each component in gel electrophoresis are indicated by plus and minus signs, respectively. Profiles that vary depending on the isolates are indicated by V.
b
The nucleotide sequences of the 493-bp and the 337-bp fragments are 99% identical at bp 1 to 337 in the direction from pS48 to pDGSL313-1.
c
The nucleotide sequences of the 450-bp and the 299-bp fragments are 99% identical at bp 1 to 299 in the direction from pS48 to pDGSL313-1.