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Development of prechallenge anti-Env binding antibody (IgG) in vaccinated macaques. The dotted line indicates the limit of detection (1:50 dilution); values below this limit were assigned values of 10. Arrows indicate times of vaccinations. D, DNA; P, particle; V, recombinant vaccinia virus. Endpoint titer is the reciprocal of the highest dilution at which the optical density is twice that of preimmune sera at the corresponding dilution.
