TABLE 1.
Plasmids used in DNA vaccine
| Plasmid | Gene product(s) | Source virus | Assay(s) showing in vitro expressionc | Antibody response in micea |
|---|---|---|---|---|
| pV1Jns:89.6Penv160OPTcleavable | Env (gp160) | SHIV89.6P | IFA, Western blotting, sCD4 binding | 5/5 to gp160 |
| pCIneo89.6 | Env (gp160) | SHIV89.6 | IFA, cell fusion, Western blotting, sCD4 binding | 2/20 to gp160 |
| pEMC*896 | Env (gp120) | SHIV89.6 | Western blotting, sCD4 binding | 5/5 to gp160 |
| pC3a896gpe | Gag | SHIV89.6 | Western blotting, ELISA | 2/5 to Gag-Pol |
| Pol | Western blotting | NT | ||
| Tat | cMAGI assay | NT | ||
| Env (gp160) | IFA, cell fusion, sCD4 binding | 2/5 to gp160 | ||
| Vif, Vpr, Vpv, Rev | NTb | NT | ||
| pVIR SIV OPT GAG | Gag | SHIV89.6 | Western blotting, ELISA | NT |
| pC3a Nef | Nef | SHIV89.6 | Western blotting | NT |
| All 6 mixed | 5/5 to gp160 | |||
| 2/5 to Gag-Pol |
a
Number of mice responding/total number of mice.
b
NT, not tested.
c
IFA, immunofluorescence assay; sCD4, soluble CD4.