TABLE 2.

Cellular immunity measured by IFN-γ ELISpot assay

Group	Animal	Resulta for viral antigen:
		Gag				Env				Pol				Accessory peptide
		Primeb	Wk 0c	Wk 2	Wk 4	Prime	Wk 0	Wk 2	Wk 4	Prime	Wk 0	Wk 2	Wk 4	Prime	Wk 0	Wk 2	Wk 4
Control	J96258	—d	2	NDe	50	—	0	ND	90	—	2	ND	0	—	2	ND	0
	J97167	—	5	10	25	—	5	0	40	—	5	35	5	—	15	0	25
	J97200	—	0	0	5	—	15	5	5	—	5	0	0	—	0	0	0
	J98071	—	0	5	10	—	0	10	0	—	0	0	10	—	0	0	0
	K96166	—	20	120f	70	—	0	30	0	—	0	0	5	—	0	5	150
	K97107	—	0	100	55	—	5	0	10	—	0	5	10	—	0	20	0
DNA-DNA	A98069	0	5	320	160	10	5	0	60	0	0	ND	0	5	10	ND	30
	J97172	0	0	25	50	0	5	75	90	0	5	430	0	0	5	145	0
	J98124	0	0	75	100	0	0	560	0	0	0	0	20	5	0	5	5
	K97246	5	0	225	90	15	5	890	75	0	0	35	0	0	0	55	100
	K98220	0	0	60	0	5	0	345	25	0	5	25	0	0	0	630	0
	T97232	0	0	300	0	0	20	15	10	0	0	0	0	0	0	35	0
DNA-particle	J97156	0	10	ND	0	0	0	ND	0	0	0	ND	0	0	0	ND	0
	J97183	0	10	5	5	0	10	70	0	0	5	0	0	0	5	25	0
	J97205	0	15	595	120	0	0	180	0	0	0	0	0	0	5	35	0
	L96018	10	160	60	595	15	30	345	45	0	35	25	70	10	10	630	30
	T98098	5	0	ND	30	0	0	ND	10	10	0	ND	10	5	0	ND	15
	T98108	0	0	55	0	0	0	0	0	0	0	15	10	0	0	305	20
DNA-vaccinia virus	A98061	5	398	1,090	230	20	362	500	220	15	0	1,300	0	0	0	15	70
	J97240	0	90	910	180	0	300	130	1,485	0	0	10	15	0	0	0	0
	K97110	0	1,018	0	20	0	1,315	110	45	0	0	0	0	0	0	170	235
	K98097	0	10	705	445	0	15	670	520	0	0	0	0	0	15	0	0
	K98099	40	40	ND	10	65	145	ND	1,595	10	0	ND	0	55	0	ND	10
	L97191	10	325	875	30	0	255	70	50	0	0	0	0	0	0	205	35
Vaccinia virus-DNA	J97168	0	25	100	145	10	15	0	25	10	0	0	25	10	10	5	65
	J97266	0	20	440	235	55	440	205	230	0	0	0	0	5	0	0	0
	K97129	10	210	430	185	10	635	5	925	0	22	0	5	0	10	0	110
	K98157	0	10	230	150	10	15	1,425	10	0	0	15	10	0	0	95	0
	L98152	215	85	815	280	35	35	455	10	5	5	15	0	0	0	10	10
	Z96290	310	90	70	5	210	575	470	15	90	0	0	5	135	0	5	5

^aSpot-forming cells/10⁶ PBMC measured by IFN-γ ELISpot with pools of overlapping peptides.

^bPrime, samples taken after 3 doses of DNA or 2 doses of recombinant vaccinia virus.

^cWk 0, day of challenge (3 weeks after the last boosting dose).

^d—, Not applicable.

^eND, not determined.

^fNumbers in boldface type are >2 times the background for that animal and >40 spot-forming cells/10⁶ PBMC.