TABLE 2.
Effects of stress conditions on growth rates of wild-type B05.10 and ΔBccrz1 strainsa
| Type of stress | Growth medium | Colony diam (mm) (% of control)
|
|||
|---|---|---|---|---|---|
| WT B05.10- MgCl2 | ΔBccrz1- MgCl2 | WT B05.10 + MgCl2 | ΔBccrz1 + MgCl2 | ||
| Control | CM | 67.0 ± 4.6 | 32.5 ± 10.8 | 70.2 ± 3.4 | 70.1 ± 4.7 |
| Cell wall | +2 mg/ml CFWb | 35.0 ± 7.8 (52) | 27.4 ± 2.3 (84) | 53.3 ± 6.3 (76) | 56.6 ± 2.8 (81) |
| +2 mg/ml Congo red | 49.1 ± 1.3 (73) | 18.8 ± 1.4 (58) | 69.0 ± 3.2 (98) | 70.6 ± 2.5 (101) | |
| Membrane | +0.02% SDS | 41.7 ± 2.1 (62) | 7.5 ± 1.4 (23) | 44.0 ± 3.1 (63) | 13.4 ± 2.9 (19) |
| +0.02% Triton X | 11.7 ± 1.1 (17) | 7.2 ± 0.5 (22) | 13.1 ± 1.3 (19) | 11.9 ± 1.4 (17) | |
| +5 μg/ml fluconazole | 46.4 ± 2.8 (69) | 9.2 ± 1.4 (28) | 48.4 ± 2.8 (69) | 56.2 ± 1.4 (80) | |
| Control | CM | 73.9 ± 1.9 | 42.6 ± 11.1 | 63.1 ± 3.4 | 62.1 ± 2.0 |
| pH | pH 3 | 54.4 ± 1.4 (74) | 6.9 ± 1.1 (16) | 31.9 ± 3.3 (51) | 8.9 ± 1.1 (14) |
| pH 9 | 45.3 ± 2.0 (61) | 8.9 ± 0.6 (21) | 34.4 ± 1.6 (55) | 34.8 ± 4.5 (56) | |
| Osmotic | +1.2 M sorbitol | 64.8 ± 1.1 (88) | 47.2 ± 1.1 (111) | 53.1 ± 1.0 (84) | 49.1 ± 2.2 (79) |
| +1 M NaCl | 25.5 ± 1.9 (35) | 13.6 ± 0.5 (32) | 29.9 ± 1.4 (47) | 24.7 ± 1.9 (40) | |
| Ionic | +30 mM LiCl | 33.4 ± 2.0 (45) | 5.0 ± 0.0 (12) | 64.3 ± 2.5 (102) | 61.5 ± 1.8 (99) |
| +20 mM MnCl2 | 17.8 ± 4.3 (24) | 42.6 ± 4.7 (100) | 46.4 ± 2.1 (74) | 47.9 ± 1.6 (77) | |
| +400 mM CaCl2 | 59.2 ± 2.4 (80) | 7.1 ± 2.7 (17) | 53.6 ± 2.0 (85) | 12.4 ± 5.7 (20) | |
| Oxidative | +10 mM H2O2 | 37.9 ± 1.8 (51) | 5.0 ± 0.0 (12) | 44.6 ± 1.0 (71) | 14.4 ± 5.6 (23) |
| +500 μM menadione | 39.1 ± 2.6 (53) | 34.8 ± 1.1 (82) | 37.4 ± 3.3 (59) | 41.4 ± 2.0 (67) | |
a
Strains were grown for 3 days on CM (with [+] or without [−] 67 mM MgCl2) containing the stressors indicated. The data are means of six colonies ± standard deviations. WT, wild type.
b
CFW, calcofluor white.