TABLE 3.
Contig name (EST database)b | Other name | B. cinerea B05.10 annotation | BlastX protein (organism), E value | Effecta of:
|
||
---|---|---|---|---|---|---|
Δbcg1 | CsA | ΔBccrz1 | ||||
Secondary metabolism | ||||||
Contig 867 | CND5/Bcbot1 | BC1G_16062.1 | CND5p (Botryotinia fuckeliana), 0.0 | ↓ | ↓ | ↓ |
Contig 1410 | P450-13 | BC1G_16061.1 | Isotrichodermin C-15 hydroxylase (Fusarium sporotrichioides), 3e−42 | ↓ | ↓ | |
Contig 1543 | CND15 | CND15p (B. fuckeliana), 8e−76 | ↓ | ↓ | ↓ | |
Contig 1214 | CND11 | BC1G_16147.1 | CND11p (B. fuckeliana), 0.0 | ↓ | ↓ | ↓ |
Contig 1506 | PKS6 | BC1G_16086.1 | Lovastatin nonaketide synthase (LNKS) (Aspergillus terreus), 0.0 | ↓ | ↓ | ↓ |
OxR | Enoyl reductase (A. terreus), 5e−47 | ↓ | ↓ | ↓ | ||
Contig 2208 | P450-1 (SSHG02) | BC1G_16085.1 | Cytochrome P450 monooxygenase (Penicillium paxilli), 5e−18 | ↓ | ↓ | ↓ |
Contig 662 | P450-2 (SSHE10) | BC1G_16084.1 | P450 monooxygenase 1 (Phoma betae), 3e−73 | ↓ | ↓ | ↓ |
045_A09 | MO1 | BC1G_16083.1 | Flavin-containing monooxygenase (Burkholderia ambifaria), 5e−67 | ↓ | ↓ | ↓ |
ORF1 | Phenylcoumaran benzylic ether reductase (Pinus taeda), 1e−04 | ↓ | ↓ | ↓ | ||
Contig 1158 | SSHB06 | Cytochrome P450 (Gibberella fujikuroi), 4e−40 | ↓ | ↓ | ↓ | |
Contig 2014 | CND14 | BC1G_03920.1 | Aldo-keto reductase (A. fumigatus Af293), 9e−119 | ↓ | ↓ | ↓ |
Contig 866 | 44-B02 | BC1G_16357.1 | Short chain dehydrogenase (Rhizobium sp. strain NGR234), 6e−35 | ↓ | ↓ | ↓ |
Contig 260 | 47-B01 | BC1G_16037.1 | Hydroxylase involved in salicylate metabolism (Acinetobacter), 9e−41 | ↓ | ↓ | ↓ |
Contig 1489 | 53-H10 | BC1G_04230.1 | Tetrahydroxynaphthalene reductase (Chaetomium globosum), 9e−127 | ↓ | ↓ | ↓ |
Other proteins | ||||||
Contig 1284 | 105-F11 | BC1G_02744.1 | 6-Phosphogluconate dehydrogenase (B. fuckeliana), 0.0 | ↑ | ↑ | ↑ |
Contig 1611 | CND6 | BC1G_05989.1 | ATP-citrate synthase subunit 1 (Sordaria macrospora), 0.0 | NA | ↓ | ↓ |
CND16 | BC1G_05991.1 | ATP citrate lyase subunit 2 (S. macrospora), 0.0 | ↓ | ↓ | ↓ | |
Contig 1746 | CND9 | BC1G_11550.1 | Mannitol-1-phosphate 5-dehydrogenase-like protein, | − | ↓ | ↓ |
Contig 1123 | BcoahA | BC1G_03473.1 | Oxaloacetate acetylhydrolase OAHA (B. fuckeliana), 1e−79 | ↓ | NA | ↓ |
Contig 1490 | Bcglyox1 | BC1G_01204.1 | Glyoxal oxidase (B. fuckeliana), 0.0 | ↓ | NA | ↓ |
Contig 802 | BC1G_12319.1 | Formate dehydrogenase (Neurospora crassa), 0.0 | − | NA | ↓ | |
Contig 1505 | BC1G_14217.1 | Acetyl-CoA hydrolase (B. fuckeliana),1e−137 | ↓ | NA | ↓ | |
Contig 944 | BC1G_03468.1 | Kynureninase (Aspergillus clavatus), 5e−39 | ↓ | NA | ↓ | |
Contig 556 | BC1G_05887.1 | Kynurenine 3-monooxygenase (A. clavatus), 3e−125 | ↓ | NA | ↓ | |
Contig 1324 | 13-H05 | BC1G_02025.1 | NADP-dependent alcohol dehydrogenase (B. fuckeliana), 0.0 | ↑ | ↑ | ↑ |
Contig 1337 | BC1G_14030.1 | 1,3-Beta-glucanosyltransferase gel4 (A. fumigatus), 1e−155 | ↑ | NA | ↓ | |
248_D11 | CND12 | BC1G_00803.1 | Calcium ion transporter VCX1 (A. fumigatus Af293), 6e−09 | − | ↓ | ↓ |
Contig 934 | SSH26/Bcacp1 | BC1G_14153.1 | Acidic protease 1 (B. fuckeliana), 1e−120 | ↓ | NA | ↑ |
Contig 902 | BC1G_03070.1 | Family A1 protease (Phanerochaete chrysosporium), 2e−13 | ↓ | NA | ↑ | |
Contig 1515 | BC1G_01286.1 | Carboxypeptidase 4 (A. fumigatus), 4e−168 | ↓ | NA | ↑ | |
Contig 783 | bcmp1 | BC1G_09180.1 | Penicillolysin (Penicillium citrinum), 5e−13 | ↓ | NA | ↑ |
Contig 667 | CND2/bcpg1 | BC1G_11143.1 | Endopolygalacturonase 1 (B. fuckeliana), 4e−88 | − | ↓ | ↓ |
Contig 1387 | CND7 | BC1G_00232.1 | Chitinase (A. fumigatus), 4e−8 | − | ↓ | ↓ |
Contig 999 | 119-G07 | BC1G_02364.1 | Beta-glucosidase (A. fumigatus), 4e−71 | − | ↓ | ↓ |
Contig 2409 | CND1 | BC1G_08931.1 | Gas1-like protein (Monacrosporium haptotylum), 1e−27 | ↓ | ↓ | ↓ |
Contig 1290 | BC1G_13581.1 | MAS1 protein (Magnaporthe grisea), 3e−105 | ↓ | NA | ↓ | |
Contig 1310 | BC1G_05503.1 | CipC-like antibiotic response protein (A. fumigatus), 6e−17 | ↓ | NA | ↓ | |
Contig 1571 | BC1G_08500.1 | NUC-2 (N. crassa), 3e−128 | − | NA | ↓ | |
Contig 2454 | BC1G_05251.1 | Cyanovirin-N family protein (Neosartorya fischeri), 3e−13 | ↓ | NA | ↓ | |
Others of unknown function | ||||||
Contig 1154 | CND3 | BC1G_10379.1 | Hypothetical protein SNOG_14859 (Phaeosphaeria nodorum), 5e−55 | ↓ | ↓ | ↓ |
Contig 2449 | CND4 | BC1G_13779.1 | No significant homologue | − | ↓ | ↓ |
Contig 1719 | BC1G_03481.1 | Hypothetical protein SNOG_00115 (P. nodorum), 5e−48 | ↑ | NA | ↓ | |
Contig 1002 | BC1G_07825.1 | No significant homologue | ↓ | NA | ↓ | |
Contig 905 | 104-H02 | No significant homologue | ↓ | ↑ | ↑ | |
Contig 1236 | BC1G_16143.1 | Hypothetical protein AN3674.2 (A. nidulans), 1e−41 | ↑ | NA | ↑ | |
Contig 1783 | BC1G_12002.1 | No significant homologue | ↑ | NA | ↓ | |
Contig 2105 | BC1G_04368.1 | No hit found | − | NA | ↓ |
Differential expression of the indicated genes in the Δbccrz1 mutant was found by a macroarray approach (wild-type B05.10 versus ΔBccrz1 mutant) and confirmed by Northern blot analyses. The expression patterns of the BcCRZ1-dependent genes in the Δbcg1 mutant and under cyclosporine treatment, mimicking a calcineurin deletion, are included (61, 75). Gene expression is described as follows: the gene is up-regulated (↑) or down-regulated (↓) in the mutant/after cyclosporine treatment in comparison to the wild type (control) or expression is not affected (−). CsA, cyclosporine A; NA, not applicable.
EST, expressed sequence tags (see Materials and Methods).