TABLE 3.

BcCRZ1-dependent genes

Contig name (EST database)b	Other name	B. cinerea B05.10 annotation	BlastX protein (organism), E value	Effecta of:
				Δbcg1	CsA	ΔBccrz1
Secondary metabolism
Contig 867	CND5/Bcbot1	BC1G_16062.1	CND5p (Botryotinia fuckeliana), 0.0	↓	↓	↓
Contig 1410	P450-13	BC1G_16061.1	Isotrichodermin C-15 hydroxylase (Fusarium sporotrichioides), 3e−42	↓	↓
Contig 1543	CND15		CND15p (B. fuckeliana), 8e−76	↓	↓	↓
Contig 1214	CND11	BC1G_16147.1	CND11p (B. fuckeliana), 0.0	↓	↓	↓
Contig 1506	PKS6	BC1G_16086.1	Lovastatin nonaketide synthase (LNKS) (Aspergillus terreus), 0.0	↓	↓	↓
	OxR		Enoyl reductase (A. terreus), 5e−47	↓	↓	↓
Contig 2208	P450-1 (SSHG02)	BC1G_16085.1	Cytochrome P450 monooxygenase (Penicillium paxilli), 5e−18	↓	↓	↓
Contig 662	P450-2 (SSHE10)	BC1G_16084.1	P450 monooxygenase 1 (Phoma betae), 3e−73	↓	↓	↓
045_A09	MO1	BC1G_16083.1	Flavin-containing monooxygenase (Burkholderia ambifaria), 5e−67	↓	↓	↓
	ORF1		Phenylcoumaran benzylic ether reductase (Pinus taeda), 1e−04	↓	↓	↓
Contig 1158	SSHB06		Cytochrome P450 (Gibberella fujikuroi), 4e−40	↓	↓	↓
Contig 2014	CND14	BC1G_03920.1	Aldo-keto reductase (A. fumigatus Af293), 9e−119	↓	↓	↓
Contig 866	44-B02	BC1G_16357.1	Short chain dehydrogenase (Rhizobium sp. strain NGR234), 6e−35	↓	↓	↓
Contig 260	47-B01	BC1G_16037.1	Hydroxylase involved in salicylate metabolism (Acinetobacter), 9e−41	↓	↓	↓
Contig 1489	53-H10	BC1G_04230.1	Tetrahydroxynaphthalene reductase (Chaetomium globosum), 9e−127	↓	↓	↓
Other proteins
Contig 1284	105-F11	BC1G_02744.1	6-Phosphogluconate dehydrogenase (B. fuckeliana), 0.0	↑	↑	↑
Contig 1611	CND6	BC1G_05989.1	ATP-citrate synthase subunit 1 (Sordaria macrospora), 0.0	NA	↓	↓
	CND16	BC1G_05991.1	ATP citrate lyase subunit 2 (S. macrospora), 0.0	↓	↓	↓
Contig 1746	CND9	BC1G_11550.1	Mannitol-1-phosphate 5-dehydrogenase-like protein,	−	↓	↓
Contig 1123	BcoahA	BC1G_03473.1	Oxaloacetate acetylhydrolase OAHA (B. fuckeliana), 1e−79	↓	NA	↓
Contig 1490	Bcglyox1	BC1G_01204.1	Glyoxal oxidase (B. fuckeliana), 0.0	↓	NA	↓
Contig 802		BC1G_12319.1	Formate dehydrogenase (Neurospora crassa), 0.0	−	NA	↓
Contig 1505		BC1G_14217.1	Acetyl-CoA hydrolase (B. fuckeliana),1e−137	↓	NA	↓
Contig 944		BC1G_03468.1	Kynureninase (Aspergillus clavatus), 5e−39	↓	NA	↓
Contig 556		BC1G_05887.1	Kynurenine 3-monooxygenase (A. clavatus), 3e−125	↓	NA	↓
Contig 1324	13-H05	BC1G_02025.1	NADP-dependent alcohol dehydrogenase (B. fuckeliana), 0.0	↑	↑	↑
Contig 1337		BC1G_14030.1	1,3-Beta-glucanosyltransferase gel4 (A. fumigatus), 1e−155	↑	NA	↓
248_D11	CND12	BC1G_00803.1	Calcium ion transporter VCX1 (A. fumigatus Af293), 6e−09	−	↓	↓
Contig 934	SSH26/Bcacp1	BC1G_14153.1	Acidic protease 1 (B. fuckeliana), 1e−120	↓	NA	↑
Contig 902		BC1G_03070.1	Family A1 protease (Phanerochaete chrysosporium), 2e−13	↓	NA	↑
Contig 1515		BC1G_01286.1	Carboxypeptidase 4 (A. fumigatus), 4e−168	↓	NA	↑
Contig 783	bcmp1	BC1G_09180.1	Penicillolysin (Penicillium citrinum), 5e−13	↓	NA	↑
Contig 667	CND2/bcpg1	BC1G_11143.1	Endopolygalacturonase 1 (B. fuckeliana), 4e−88	−	↓	↓
Contig 1387	CND7	BC1G_00232.1	Chitinase (A. fumigatus), 4e−8	−	↓	↓
Contig 999	119-G07	BC1G_02364.1	Beta-glucosidase (A. fumigatus), 4e−71	−	↓	↓
Contig 2409	CND1	BC1G_08931.1	Gas1-like protein (Monacrosporium haptotylum), 1e−27	↓	↓	↓
Contig 1290		BC1G_13581.1	MAS1 protein (Magnaporthe grisea), 3e−105	↓	NA	↓
Contig 1310		BC1G_05503.1	CipC-like antibiotic response protein (A. fumigatus), 6e−17	↓	NA	↓
Contig 1571		BC1G_08500.1	NUC-2 (N. crassa), 3e−128	−	NA	↓
Contig 2454		BC1G_05251.1	Cyanovirin-N family protein (Neosartorya fischeri), 3e−13	↓	NA	↓
Others of unknown function
Contig 1154	CND3	BC1G_10379.1	Hypothetical protein SNOG_14859 (Phaeosphaeria nodorum), 5e−55	↓	↓	↓
Contig 2449	CND4	BC1G_13779.1	No significant homologue	−	↓	↓
Contig 1719		BC1G_03481.1	Hypothetical protein SNOG_00115 (P. nodorum), 5e−48	↑	NA	↓
Contig 1002		BC1G_07825.1	No significant homologue	↓	NA	↓
Contig 905	104-H02		No significant homologue	↓	↑	↑
Contig 1236		BC1G_16143.1	Hypothetical protein AN3674.2 (A. nidulans), 1e−41	↑	NA	↑
Contig 1783		BC1G_12002.1	No significant homologue	↑	NA	↓
Contig 2105		BC1G_04368.1	No hit found	−	NA	↓

^aDifferential expression of the indicated genes in the Δbccrz1 mutant was found by a macroarray approach (wild-type B05.10 versus ΔBccrz1 mutant) and confirmed by Northern blot analyses. The expression patterns of the BcCRZ1-dependent genes in the Δbcg1 mutant and under cyclosporine treatment, mimicking a calcineurin deletion, are included (61, 75). Gene expression is described as follows: the gene is up-regulated (↑) or down-regulated (↓) in the mutant/after cyclosporine treatment in comparison to the wild type (control) or expression is not affected (−). CsA, cyclosporine A; NA, not applicable.

^bEST, expressed sequence tags (see Materials and Methods).