Pathogenicity assay with the A. brassicicola wild type, aso1 mutants, and ectopic transformant. (A, C, and E) Inoculated, abaxial surface at 6, 12 and 18 days postinfection; (B and D) adaxial (uninoculated) surface of the same cabbage leaves at 6 and 12 days postinfection; (F) wild type (wt) and aso1 lesions, at a magnification of ×2. Panel A and C isolate numbers are identified as follows: wild type, 1, 8, 13, and 15; aso1-10a, 2, 7, 14, and 17; aso1-11a, 5, 9, 11, and 19; aso1-14a, 3, 10, 16, and 18; aso1-7E (ectopic), 4, 6, 12, and 20.