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. 2008 Feb 15;7(4):610–618. doi: 10.1128/EC.00017-08

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FIG. 1. — Subcellular localization of citrate synthase and carnitine acetyltransferase in S. cerevisiae and C. albicans. Nycodenz density gradient analysis of the organellar pellet fraction obtained from wild-type C. albicans (A) and wild-type S. cerevisiae (B) grown on rich oleic acid medium. Enzyme assays were performed on the gradient fractions to determine the distribution of the peroxisomal marker enzyme 3-hydroxyacyl-CoA dehydrogenase (3HAD), the mitochondrial marker enzyme fumarase, and Cat. Activities are given as percentages of the total activity present in the whole gradient. Relative Cat activities were determined for the peroxisomal and mitochondrial peak fractions of both gradients, 4,088.8 nmol/min/mg for the C. albicans peroxisomal peak fraction (3), 445.6 nmol/min/mg for the mitochondrial peak fraction (8), 82.2 nmol/min/mg for the S. cerevisiae peroxisomal peak fraction (4), and 122.6 nmol/min/mg for the mitochondrial peak fraction (8). The localization of citrate synthase in the gradients was determined by immunoblotting with an antibody directed against S. cerevisiae citrate synthase 1 (Cit1p).