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The cat2 null mutant is unable to grow on nonfermentable carbon sources. Glucose-grown cultures (about 2.7 × 107 cells/ml) of the indicated strains were serially diluted (1:10), and 4 μl of each dilution was spotted on a minimal plate containing glucose, oleate, acetate, or ethanol as the sole carbon source. Pictures were taken after 3 to 7 days of incubation at 28°C.
