FIG. 1.

Diagram of the cpDNA segment in the transforming plasmids pIF24 and pOF28. The shaded boxes represent the chloroplast-borne genes of C. reinhardtii, and the arrows indicate the directions of their transcription. Two triangles represent the insertions in the transforming plasmid construct: the aadA cassette is the selectable marker that was inserted between two chloroplast genes; the IF24 (24-bp) and OF28 (28-bp) SSRs were inserted at the sixth codon position of the rbcL gene in plasmids pIF24 and pOF28, respectively. The enzymes used for cloning are indicated. The size of the reporter construct was 8.1 kb, including the pUC8 vector.