FIG. 7.

PCR analysis to identify meiotic progeny carrying both the cpDNA microsatellite reporter and the E. coli ΔN42RecA gene. (A) PCR screening of spectinomycin-resistant progeny for the microsatellite reporter. PCR products from 13 progeny (1 to13) and the wild type (WT) were amplified with the rbcL5 and rbcLmid primers to allow visualization of the region containing the microsatellite. (B) PCR products indicating the presence of the ΔN42RecA gene. The lanes are labeled as in panel A. The faint lower band is derived from excess oligonucleotides in the reaction mixture.