TABLE 2.
Mixing experimenta
| Expected no. of colonies of photosynthetic strain | Observed no. of green colonies in bleached lawn of cells from strain:
|
|||
|---|---|---|---|---|
| 18-7G
|
OF-28
|
|||
| CC-124 | IF24 | CC-124 | IF24 | |
| 50 | 131 ± 7 | 53 ± 5 | 97 ± 7 | 27 ± 2 |
| 5 | 16 ± 5 | 6 ± 1 | 20 ± 0 | 6 ± 1 |
| 0.5 | 3 ± 1 | 0 | 2 ± 2 | 0 |
a
Aliquots from a 10-fold dilution series of the photosynthetically competent lines CC-124 (wild type) or IF24 (8-codon insertion in rbcL) were added to nonphotosynthetic cells (1×106 cells/ml) carrying a point mutation in rbcL (18-7G) or an out-of-frame insertion (OF28). The expected colony numbers are based on hemocytometer cell counts. Photosynthetic colonies were scored after 5 to 8 days.