FIG. 1.

Arrest of Giardia cell cycle with aphidicolin and nocodazole. (A and B) Subjecting a control culture with a 5:1 ratio of G₂ to G₁ cells (A) to treatment with 6 μM aphidicolin (Aph) for 6 h results in very loose cell cycle arrest in G₁, with a significant number of cells in G₂ (B). (C) After the removal of aphidicolin, cultures were exposed to 100 nM nocodazole (Noc) for 2 h, resulting in very tight G₂ arrest.