FIG. 2.

TcoCBc1, TcoCBc6, and TcoCBs12 expression strategies and successfully purified recombinant proteases. The prepeptide (pre), propeptide (pro), and catalytic regions are highlighted. The occluding loop structure identified in cathepsin B-like proteases from other species is framed, and the deduced amino acids of the active site are indicated in large boldface letters. Potential N-glycosylation sites and mutations performed are indicated by small letters at the top of each protein structure. N-terminal sequencing of the matured forms revealed two maturation sites in TcoCBc1, GAFR (major) and FTEE (minor), one for TcoCBc6 (VSEAK), and one for TcoCBs12 (RTKLP).