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. 2008 Feb 15;7(4):684–697. doi: 10.1128/EC.00405-07

FIG. 4.

FIG. 4.

pH activity profiles of active recombinant proteases TcoCBc1 and TcoCBc6 with endopeptidase (Z-Phe-Arg-AMC and Z-Arg-Arg-AMC; broken lines) and carboxypeptidase (Abz-Phe-Arg-Ala-Lys*-OH; continuous lines) fluorogenic substrates. Recombinant proteases were assayed in AMT (acetate-MES-Tris) buffers across the indicated pH range in the presence of 5 mM DTT at 37°C after 10 min of preincubation in reaction buffers. •, TcoCBc1 activity with Z-Phe-Arg-AMC; ▪, TcoCBc1 activity with Z-Arg-Arg-AMC; ▴, TcoCBc1 activity with Abz-Phe-Arg-Ala-Lys*-OH; and ⊠, TcoCBc6 activity with Abz-Phe-Arg-Ala-Lys*-OH.