TABLE 2.
Sensitivity of T. congolense recombinant TcoCBc1 and C2 to protease inhibitors in vitroa
| Inhibitor | % Residual activity of:
|
|
|---|---|---|
| TcoCBc1 | C2 | |
| Leupeptin | 100 | 0 |
| Ac-Leu-Val-lysinal-CHO | 85 | 0 |
| Cystatin | 77 | 2 |
| Chymostatin | 100 | 0 |
| E-64 | 0 | 0 |
| CA074 | 0 | 94 |
| Z-Phe-Gly-NHO-Bz-pMe | 13 | 88 |
| Buffer lacking DTT | 0 | 0 |
a
Protease at a concentration of 3.5 nM was incubated with 350 nM of each inhibitor for 1 h at 37°C in 30 mM Na-acetate buffer, pH 4.5, containing 5 mM DTT and 5 mM EDTA. Assays were performed in the same buffer, and the initial velocities of the hydrolysis of Z-Phe-Arg-AMC were determined and compared to the initial velocity in the absence of inhibitor. The values are the means from three replicates; the standard error was always less than 5%.