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Polysome profile analysis in wt A. fumigatus. (Top) Polysome profile of wt A. fumigatus germlings cultured in rich medium at 37°C. The profile shows a protein peak (P), followed by peaks representing free 40S and 60S subunits, the 80S monosome and polysomes representing two or more ribosomes per mRNA. (Middle) SYBR green staining of rRNA in each polysome fraction (10 fractions). (Bottom) Northern blot showing hybridization of RNA in each fraction to an A. fumigatus β-actin probe.
