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. 2008 Feb 22;7(4):575–583. doi: 10.1128/EC.00412-07

FIG. 5.

FIG. 5.

Validation of microarray data by real-time PCR. Two mRNAs that showed increased abundance in the ΔcgrA mutant relative to the wt following the shift from 30°C to 37°C by microarray analysis were confirmed by qRT-PCR. Ct values were calculated for each gene 80 min following a shift from 30°C to 37°C. Relative expression values were normalized to the 0-min time point and to the reference gene fks as described in Materials and Methods.