FIG. 1.

PorB induces PTK activation and Erk1/2 phosphorylation. B cells were isolated from either C57BL/6 or TLR2^−/− mice and treated for the indicated times with medium alone (M), 10 μg of PorB/ml (P), 100 ng of N. meningitidis LPS/ml (L), or 10 μg of anti-IgM/ml plus a 1:1,000 dilution of CD40L (μ). Whole-cell lysates were generated and subjected to Western blotting for either phosphorylated tyrosine (P-Tyr) (A) or phosphorylated Erk1/2 (P-Erk) (B). Immunoblots were stripped and reprobed for total Erk1/2 (T-Erk) as a loading control. Molecular size markers are shown on the left of panel A. 5′, 15′, and 30′, 5, 15, and 30 min.