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. 2008 Feb 6;15(4):598–606. doi: 10.1128/CVI.00472-07

FIG. 1.

FIG. 1.

Confirmation of the MURD-specific deletions affecting esxB (CFP-10) and esxA (ESAT-6) in an M. ulcerans worldwide strain collection. (A) Schematic view of an alignment of M. marinum M and M. ulcerans Agy99 genomic sequences displayed by the Artemis comparison tool (9). Regions of conformity are shown in parallel gray plains, an inverted DNA segment is depicted as an inverted surface, and white areas represent unique sequences like MURD152, which is present only in M. marinum M and is deleted from M. ulcerans Agy99. Indicated are the genes esxB and esxA and the PCR primers (CH1 through CH4) used for this experiment. (B) PCR products of 162 bp or 1,712 bp proved the MURD152 deletion of 2.8 kb and the MURD4 deletion of 12 kb, respectively.