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Localization of the two esxB-esxA clusters in the genomes of strains of the M. ulcerans ancestral lineage. Positions of the corresponding primers are indicated for the PCR product of the esxB-esxA cluster, where CH1 and CH2 correspond to sequences within the CDSs of both locations, and of the slightly larger PCR products amplified with flanking primers specific for either MURD152 or MURD4 (Table 1).
