FIG. 3.
Enumeration of NcSRS2-specific PBMC by IFN-γ ELISPOT in cattle following NcSRS2 DNA and NcSRS2-lipopeptide immunization. There were significant differences between immunized and control groups (*, P ≤ 0.05; Student's t test) for LP 20-21, LP 34-36, NSo (WSU), and NcSRS2 peptide pool. (A) Results from Washington State University. NSo was used only at WSU. (B) Results from Kimron Veterinary Institute. NcSRS2 Vaccinate, experimental group received VR-NcSRS2PI plus LP 20-21 and LP 34-36 plus adjuvant; Control, group received VR-1055 vector alone plus adjuvant alone; RPMI, medium alone (negative control); ConA, concanavalin A mitogen (positive control); SRS2 peptide pool, NcSRS2 peptide pool (Staska et al. [39]). ELISPOT values (y axis), number of IFN-γ-secreting cells per 1 × 106 PBMC. Error bars, 1 standard deviation. For data on individual animals, see Table 3.