TABLE 4.

ELISA titers for recombinant fragment At before and after methylation

Patient serum samplea	Titer of antibody against Atb
	IgG		IgM
	rAt	mrAt	rAt	mrAt
21s1	800 (1,600)	400	1,600 (400)	400
21s2	0 (400)	0	100 (0)	100
40s1	800 (6,400)	800	100 (200)	100
55s1	800 (200)	800	800 (0)	800
61s1	800 (400)	1,600	200 (200)	200
88s1	800 (3,200)	800	400 (1600)	1,600
158s1	6,400 (25,600)	3,200	100 (0)	100
158s2	6,400 (12,800)	1,600	200 (200)	200
1935s2	200 (6,400)	12,800	6,400 (12,800)	51,200
1936s1	1,600 (6,400)	800	800 (100)	100
1936s2	1,600 (6,400)	1,600	800 (200)	100
3210s1	3,200 (800)	6,400	6,400 (400)	6,400
3210s2	1,600 (800)	1,600	800 (400)	800
4425s3	3,200 (25,600)	51,200	200 (NDc)	800
5206s1	6,400 (12,800)	6,400	100 (200)	1,600
5206s2	6,400 (12,800)	6,400	0 (100)	1,600
5507s1	400 (0)	400	0 (0)	0
5507s2	200 (400)	800	0 (0)	0
6358s1	800 (6,400)	1,600	800 (800)	6,400
9557s1	400 (12,800)	400	100 (200)	100
9557s2	1,600 (12,800)	800	100 (200)	100
78-009241s1	800 (6,400)	800	100 (100)	800
78-009241s2	1,600 (12,800)	3,200	200 (400)	1,600
016733s2	1,600 (12,800)	1,600	100 (800)	100
016733s3	1,600 (51,200)	1,600	0 (400)	100
80-062062s2	6,400 (800)	6,400	800 (1,600)	1,600
81-062767s1	3,200 (800)	6,400	400 (200)	1,600
81-062767s2	3,200 (6,400)	3,200	800 (400)	1,600
77-083661	800 (6,400)	3,200	800 (ND)	3,200
80-053680	3,200 (51,200)	12,800	1,600 (ND)	51,200
82-038629	1,600 (25,600)	6,400	800 (ND)	12,800

^aSamples with the same prefix followed by s1 and s2, or s2 and s3 were the acute- and convalescent-phase sera from the same patient. 77-083661, 80-053680, and 82-038629 are acute-phase sera.

^bNumbers in parentheses are titers obtained using full-length OmpB. The titer 0 indicates that the OD was below positive cutoff (average OD₄₀₅ of negatives [normal human sera] plus 2 standard deviations, 0.266) at a 1:100 dilution and was considered negative.

^cND, not determined.