FIG. 2.

P. aeruginosa 1244 growth in BAL fluid (A) or the lungs, livers, and spleens (B) of strain 1244 pilus-immunized and saline-immunized mice. The graphed values are medians, and error bars represent quartiles. Data are from two independent experiments (n = 4 mice per group per experiment). (A) *, P = 0.0062 for the counts in mice immunized i.n./i.n. and P = 0.0015 for the counts in mice immunized i.n./s.c. compared to the counts for the corresponding times postinfection of saline-immunized (control) animals (Mann-Whitney U test). (B) P. aeruginosa 1244 CFU counts from organ homogenates at 4, 24, or 48 h postinfection of mice immunized i.n./i.n., i.n./s.c., or with saline (control) are shown for all time points except for control mice at 48 h, as these mice were dead, making statistical analysis infeasible.