TABLE 2.
Systemic antibody responses of mice specific for P. aeruginosa O7 LPS after immunization with strain 1244 pili
| Routea | Ig class | Mean ELISA antibody titer on the indicated day after the second immunizationb
|
|||
|---|---|---|---|---|---|
| 3 | 7 | 10 | 14 | ||
| i.n./i.n. | IgG | 112 | 79.4 | 58.9 | 234 |
| IgM | 479 | 269 | 91.2 | 93.3 | |
| IgA | ND | ND | ND | ND | |
| i.n./s.c. | IgG | 166 | 269 | 135 | 123 |
| IgM | 617 | 794 | 676 | 490 | |
| IgA | ND | ND | ND | ND | |
a
The routes denoted are for the first/second immunization, in which the time between each immunization was 7 days.
b
Mean ELISA antibody titer (dilution) which gave a change in the A405 of 0.200 after 30 min. Each value shown is the mean of the individual titers from three mice. ND, none detected, as these values were below the limits of detection (A405 = 0.050).