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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Dev Cell. 2008 Feb 7;14(3):377–387. doi: 10.1016/j.devcel.2008.01.006

Figure 4. Inactivation of Sd reduces organ size and downregulates Hpo target genes.

Figure 4

(A–E) Adult eyes of wild type (A), or expressing two copies of UAS-SdRNAiN (B), two copies of UAS-SdRNAiC (C), UAS-SdRNAiN +UAS-SdRNAiC (D), or two copies of UAS-Yki-RNAiN (E) with ey-Gal4 in the presence of UAS-Dicer2. (F–G′, J–J′) Wing discs expressing UAS-GFP (F, F′), UAS-GFP +UAS-Sd-RNAiN + UAS-Sd-RNAiC (G, G′), or UAS-GFP plus two copies of UAS-Yki-RNAi (J, J′) with hh-Gal4 were immunostained to show the expression of Diap1(red) and GFP (green). GFP marked the P-compartment (arrows). (H–I′, K, K′) Wild type wing disc (H, H′), or wing discs expressing UAS-Sd-RNAiN +UAS-Sd-RNAiC (I, I′) or two copies of UAS-Yki-RNAi (K, K′) with hh-Gal4 were immunostained to show the expression of Ci (red) and ex-lacZ (blue). The A/P boundary is indicated by white lines based on Ci expression (I′, K′). Arrows indicate the P-compartment. (L–M′) Eye (L, L′) and wing (M. M′) discs containing flip-out clones expressing UAS-Sd-RNAiN +UAS-Sd-RNAiC with act>CD2>Gal4 were immunostained to show the expression of CD2 (red) and a bantam sensor (BS; green). Cells expressing Sd-RNAi transgenes were marked by the lack of CD2 expression (arrows).