Table 1.
Selected list of modulated grape transcripts.
| TDF | Primer comb. | Accession | Lenght (bp) | I/R | Annotation | Blast score Blastn/Blastx* |
| Metabolism | ||||||
| 192 | TC – CA | TC52910 | 301 | + | Sucrose synthase (Q9SLS2) | 3.12E-63 |
| 1627 | TT – CT | TC57852 | 168 | + | UDP-glucosyltransferase HRA25 (Q9FUJ6) | 2.07E-30 |
| 245 | TA – AC | TC53221 | 169 | + | Transketolase, chloroplast precursor (O20250) | 1.92E-29 |
| 18 | TT – AA | TC67193 | 127 | + | Raffinose synthase, partial (Q8VWN6) | 9.26E-21 |
| 1279 | CT – TC | TC52362 | 175 | + | Fatty acid multifunctional protein (Q9ZPI5) | 1.12E-16 |
| 134 | TC – TT | TC67104 | 390 | + | 12-oxophytodienoate-10,11-reductase 1 (Q8LAH7) | 6.19E-24 |
| 993 | CA – CA | TC60564 | 50 | + | Gibberellin 2-oxidase (Q6TN17) | 3.63E-105 |
| 215 | TT – TA | TC55722 | 216 | + | Fatty acid hydroperoxide lyase (Q9AUD8) | 5.62E-72 |
| 152 | TC – TC | Q6X5R6 | 342 | + | (Lox2) Lipoxygenase | 4.00E-39 * |
| 1036 | CC – AT | TC58112 | 349 | + | Long-chain acyl-CoA oxidase (O64894) | 5.62E-72 |
| 1225 | CT – AC | TC53311 | 359 | + | 3-ketoacyl-CoA thiolase (Q6TXD0) | 2.10E-09 |
| 893 | CA – AC | TC67959 | 104 | + | 3-beta-hydroxy-delta5-steroid dehydrogenase | 9.77E-16 |
| 347 | TA – CG | TC54708 | 181 | + | 40S ribosomal protein S16 (Q9M5L1) | 2.91E-18 |
| 1382 | CG – TA | TC58494 | 279 | + | Ribosomal L10 protein (Q874B2) | 2.62E-56 |
| 1345 | CG – AA | TC51894 | 289 | + | Ubiquitin conjugating enzyme E2 (Q42897) | 6.56E-55 |
| 594 | TG – GA | TC60588 | 186 | + | Proteasome subunit beta type 5-B precursor (Q9LIP2) | 5.35E-37 |
| 1493 | CT – GG | TC56558 | 399 | + | Gamma-glutamylcysteine synthetase (Q6F4I8) | 4.64E-35 |
| 1293 | CT – TG | TC51806 | 244 | + | Cysteine synthase (Q43317) | 3.08E-25 |
| 79 | TC – AC | TC68684 | 457 | + | Cellulose synthase-like protein D4 (Q8GUZ9) | 2.09E-96 |
| 1594 | TT – TC | TC65238 | 179 | + | Tubulin alpha chain, partial (P33629) | 4.78E-34 |
| 232 | TA – AT | TC57434 | 161 | - | Nucleotide sugar epimerase-like protein | 8.83E-12 |
| 1630 | TT – CT | Q9ZTP5 | 105 | - | Pentose-5-phosphate 3-epimerase | 5.85E-10 * |
| 1610 | TT – CA | TC54570 | 167 | - | Sedoheptulose-bisphosphatase (Q940F8) | 1.37E-27 |
| 1668 | TT – GT | TC54602 | 412 | - | Fructose-bisphosphate aldolase (Q6RUF6) | 1.47E-50 |
| 100 | TC – AG | TC54851 | 224 | - | Alpha-mannosidase (Q2R3E0) | 9.53E-43 |
| 1567 | CG – GT | TC57827 | 211 | - | Galactose dehydrogenase, (Q84LI1) | 4.13E-33 |
| 255 | TA – AG | TC52686 | 210 | - | Carbonic anhydrase (Q5NE20) | 2.18E-39 |
| 1472 | CT – GC | TC60916 | 581 | - | Glyceraldehyde-3-phosphate dehydr. B subunit | 1.61E-126 |
| 1205 | CT – AT | TC62475 | 182 | - | Lipase class 3-like (Q6K2K7) | 6.71E-33 |
| 1689 | TT – GG | TC53435 | 141 | - | B-keto acyl reductase (O24479) | 4.93E-24 |
| 670 | CA – GA | TC62496 | 145 | - | 3-hydroxy-3-methylglutaryl coenzyme A (Q8W2E3) | 6.52E-12 |
| 151 | TC – TC | Q8H539 | 358 | - | Steroid 5alpha-reductase-like protein | 1.00E-32* |
| 1239 | CT – AG | TC69679 | 98 | - | 60S ribosomal protein L19 (Q6RYC4) | 1.65E-13 |
| 138 | TC – TT | TC59193 | 99 | - | Protein translation factor SUI1 homolog 2 (Q94JV4) | 1.49E-12 |
| 308 | TA – CA | TC51783 | 621 | - | Elongation factor 1-beta 1 (Q84WM9) | 1.99E-64 |
| 823 | TC – GG | TC54220 | 542 | - | Phytocalpain (Q6SSJ2) | 3.95E-39 |
| 1174 | CC – CC | TC63107 | 115 | - | Chaperone protein dnaJ-like (Q6H3Y3) | 9.58E-18 |
| 1530 | CC – GC | CB348741 | 62 | - | F-box protein family AtFBL5 | 1.37E-12 |
| 1476 | CT – GC | CA816379 | 345 | - | Protein At3g07360 (U-box domain-containing prot.) | 2.37E-69 |
| 1389 | CG – TT | TC65574 | 203 | - | Glycin-rich protein (Q43688) | 3.13E-35 |
| 1546 | CG – GA | TC68519 | 541 | - | Expansin, complete (Q84US7) | 8.40E-112 |
| 722 | TC – CT | TC69230 | 350 | - | Cyclin D1, partial (Q8GVE0) | 1.73E-69 |
| 664 | CA – GA | TC53870 | 228 | - | Integral membrane protein-like (Q5VRH) | 7.89E-07 |
| Photosyntesis and Energy | ||||||
| 1330 | CT – CC | TC61438 | 646 | + | Cytochrome P450 monooxygenase (Q2MJ14) | 1.38E-67 |
| 123 | TC – TA | TC68921 | 64 | + | C-type cytochrome biogenesis protein (Q7XY14) | 1.08E-06 |
| 227 | TA – AT | TC62259 | 353 | - | ATP synthase B' chain, chloroplast precursor | 1.03E-66 |
| 749 | TC – CG | TC63430 | 230 | - | NADPH-cytochrome P450 oxydoreductase | 1.20E-29 |
| 520 | TG – TG | TC65998 | 102 | - | Plastocyanin, chloroplast precursor (P17340) | 6.20E-06 |
| 1247 | CT – TA | TC66316 | 365 | - | Chloroplast photosystem II 10 kDa protein (Q2PXN6) | 5.90E-58 |
| 1156 | CC – CT | TC68056 | 154 | - | NADH-plastoq. oxidoreductase subunit 7 (Q2L953) | 2.39E-26 |
| 1049 | CC – AC | TC53584 | 561 | - | Magnesium chelatase subunit, partial | 1.28E-121 |
| 31 | TT – AT | TC55659 | 163 | - | Chlorophyll A/B binding protein precursor (Q32291) | 1.79E-32 |
| 427 | TG – TA | TC57132 | 209 | - | Chlorophyll A/B binding protein, precursor (P13869) | 3.62E-42 |
| 4 | TT – AA | TC55138 | 381 | - | Photosystem I assembly protein ycf3, partial | 2.10E-78 |
| 1115 | CC – TC | TC61693 | 411 | - | Photosystem I reaction center subunit II (P12372) | 2.51E-84 |
| 1322 | CT – CT | TC66994 | 275 | - | Photosystem II reaction center (Q8W536) | 2.26E-52 |
| 161 | TC – TC | TC58978 | 146 | - | Photosystem II M protein (Q6QXV8) | 3.80E-25 |
| 1393 | CG – TT | TC58567 | 103 | - | Apocytochrome f precursor (Q68RZ3) | 4.92E-10 |
| Transport | ||||||
| 1614 | TT – CA | Q3L7K6 | 185 | + | Hexose transporter | 6.26E-08 * |
| 680 | CA – GT | TC52346 | 231 | - | Transporter-like protein (Q9LSH7) | 3.88E-47 |
| 249 | TA – AC | TC66367 | 107 | - | 14-3-3 protein (Q93XW1) | 1.13E-16 |
| 1534 | CC – GG | TC57372 | 435 | - | Nitrate transporter NRT1-2 (Q9FRU5) | 8.33E-94 |
| 1438 | CG – CC | TC65826 | 270 | - | ABC transporter-like protein (Q9FT51) | 7.38E-50 |
| 133 | TC – TT | TC62785 | 219 | - | Calcium-transporting ATPase 8, plasma membr. | 4.00E-23 |
| 662 | CA – GA | TC69251 | 302 | - | ATPase alpha subunit, complete (Q3C1H4) | 7.47E-59 |
| 1679 | TT – GC | BQ798655 | 127 | - | Syntaxin {Glycine max} | 5.39E-23 |
| 792 | TC – GT | TC51721 | 180 | - | ADP-ribosylation factor 1-like protein (Q70XK1) | 2.22E-36 |
| 62 | TT – AG | TC69827 | 195 | - | Aquaporin PIP2 (Q2HZF5) | 1.45E-20 |
| Signal transduction | ||||||
| 434 | TG – TA | TC59460 | 147 | + | Protein phosphatase 2C (Q8RVG0) | 6.61E-23 |
| 240 | TA – AC | Q52QR5 | 343 | + | NAC domain protein NAC1 | 1.70E-71* |
| 1385 | CG – TA | TC55407 | 95 | + | NAC family protein (Q2Z1Y1) | 2.32E-13 |
| 687 | CA – GT | TC70344 | 687 | + | WRKY DNA-binding protein 32 (P59583) | 1.24E-05 |
| 235 | TA – AT | TC52484 | 102 | - | SOS2-like protein kinase (Q8LK24) | 1.58E-12 |
| 1298 | CT – TG | TC60214 | 164 | - | Calcium-transporting ATPase/calmodulin binding | 2.74E-28 |
| 288 | TA – TC | TC52400 | 212 | - | Calmodulin cam-207 (Q6DN29) | 1.23E-39 |
| 1538 | CC – GG | TC66781 | 267 | - | CNGC2 (Q5D6H2) | 1.22E-47 |
| 633 | TG – GC | TC69501 | 633 | - | Calcium sensor calcineurin B-like protein (Q4W3B4) | 1.02E-34 |
| 11 | TT – AA | TC61968 | 289 | - | Protein At1g01140 (CBL-interacting protein kinase) | 7.34E-54 |
| 778 | TC – GA | TC53469 | 55 | - | MAPKK (Q66MH6) | 8.34E-07 |
| 1573 | CG – GC | TC59576 | 700 | - | Receptor-like kinase with LRR repeats (Q70AH8) | 1.44E-32 |
| 1231 | CT – AG | TC52043 | 339 | - | MADS-box transcription factor FBP29 (Q9ATE2) | 2.44E-72 |
| Secondary metabolism | ||||||
| 1539 | CC – GG | TC52853 | 419 | + | Cinnamyl alcohol dehydrogenase (Q2Z1Z0) | 2.21E-08 |
| 909 | CA – AG | TC53668 | 105 | + | Stilbene synthase [Vitis vinifera] | 5.34E-17 |
| 1249 | CT – TA | TC54354 | 273 | - | Secretory laccase (Q6TDS6) | 1.11E-23 |
| 421 | TG – AG | TC51729 | 113 | - | Caffeoyl-CoA O-methyltransferase (Q43237) | 5.87E-17 |
| 362 | TG – AA | TC53331 | 208 | - | Flavanone 3-hydroxylase-like protein, (Q9FLV0) | 2.88E-34 |
| 432 | TG – TA | TC65435 | 179 | - | Limonoid UDP-glucosyltransferase (Q9MB73) | 1.29E-32 |
| Defence response | ||||||
| 1176 | CC – CG | TC63410 | 557 | + | Beta-1,3-glucanase (Q9M563) | 5.95E-120 |
| 68 | TT – AG | TC56756 | 108 | + | Pathogenesis-related protein 10.3 (Q20BD2) | 6.31E-14 |
| 724 | TC – CT | TC56512 | 253 | - | Pollen allergen-like protein, partial {A. thaliana} | 1.09E-49 |
| 1296 | CT – TG | TC62916 | 157 | - | EXECUTER1 protein (Q93YW0) | 1.72E-27 |
| 692 | CA – GC | TC57989 | 251 | - | Avr9/Cf-9 rapidly elicited protein 276 (Q84QD7) | 3.72E-23 |
| 229 | TA – AT | TC70153 | 282 | - | Flax-inducible sequence 1 (Q40255) | 6.91E-56 |
| 1173 | CC – CC | TC51855 | 262 | - | Pto-like serine/threonine kinase (Q6W0C7) | 1.69E-16 |
| 1545 | CG – GA | TC58939 | 671 | - | Ethylene-resp. element-binding factor (Q2QDF5) | 3.13E-125 |
| 584 | TA – GC | TC62111 | 83 | - | Lipid transfer protein (Q93YX9) | 4.49E-10 |
| 889 | CA – AC | TC63540 | 305 | - | DIR1 (At5g48485) {Arabidopsis thaliana} | 2.98E-58 |
| 214 | TT – TA | TC61755 | 233 | - | Enzymatic resistance protein, complete (Q3S4G9) | 1.61E-44 |
| Response to stimulus | ||||||
| 246 | TA – AC | TC63756 | 153 | + | Metallothionein-like protein (Q3HR41) | 3.69E-27 |
| 945 | CA – TT | TC53817 | 79 | + | Thioredoxin H, (Q4U0W0) | 1.68E-08 |
| 1275 | CT – TC | TC53088 | 383 | + | Glutathione S-transferase GST 24 (Q9FQD4) | 7.96E-81 |
| 748 | TC – CG | TC54876 | 240 | + | Ferritin-3, chloroplast precursor (Q948P6) | 2.59E-47 |
| 1631 | TT – CT | Q6V7W6 | 84 | + | Class III peroxidase GvPx2b | 2.76E-04 |
| 1245 | CT – TA | TC62299 | 380 | - | Auxin-induced SAUR-like protein (Q8S351) | 1.89E-79 |
| 1562 | CG – GT | TC53184 | 397 | - | Chloroplast small heat shock protein (Q6WHC0) | 1.14E-80 |
| 781 | TC – GA | TC53791 | 232 | - | Catalase (Q7XTK9) | 4.25E-45 |
| 532 | TG – CT | TC56223 | 206 | - | Peroxiredoxin Q (Q6QPJ6) | 4.38E-37 |
I/R: induced or repressed in cDNA-AFLP experiments.