Figure 5.
Replication kinetics of subtype C molecular clones in different cell types. CD8-depleted and mitogen-activated PBMC (A) monocyte-derived macrophages (B) CN-2 cells, an Herpesvirus saimiri immortalized cell line (C) and a human brain astrocytoma cell line, GO-G-CCM (D) were infected with 500 TCID50 units of different viral molecular clones. While proliferation of D17and D24 was compared with that of Indie-C1 in all the cell types, in PBMC alone, subtype B standard clones NL4-3 and 86.9 (open symbols) were also used. Viral replication was monitored by quantifying p24 levels in the culture medium at defined time points using a commercial antigen-capture ELISA. The data presented are representative of three independent experiments. The data are presented as mean quantity of p24 released in triplicate wells ± 1 SD.