Table 2.

Oligo-nucleotide primers for the viral amplification

Amplification	Primers	HXB2 Coordinate	Sequence (5'-3')
N-term 3.0 kb fragment
First round	N558F	0001	TGGAAGGGTTAATTTACTCTAAGGAAAGGCAAGAGATCCTTG
	N453R	4820	CTATTATGTCTACTATTCTTTCCCCTGCAC
Second round	N533F	0001	GGGGGGCAGTGCGGCCGCTGGAAGGGTTAATTTACTCYMAGAAAAGRCAAG
	N502R	2878	TGAAAAATACGCGTCMCCCACATCCAGTACTGT
C-term 6.5 kb fragment
First round	N501F	1851	CCAYAAAGCACGCGTKTTGGCTGARGCAATGAG
	N476R	9719	TGCTAGAGATTTTCCACACTGACTAAAAGGGTCTGAGGTCTCTAGTT
Second round	N503F	2841	ACTGGATGTGGGKGACGCGTATTTTTCAGTTCC
	N600R	9719	GCTTCGCATCCTGCAGGTGCTAGAGATTTTCCACACTGACTAAAAGGGTC

Sequences of the reverse oligo-nucleotides have been presented as inverse complement. Restriction enzyme sites have been underlined.