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. 2008 Jan 3;177(8):861–870. doi: 10.1164/rccm.200708-1269OC

Figure 8.

Figure 8.

Cigarette smoke extract (CSE) caused post-translational modifications of sirtuin (SIRT1). (A) SIRT1 protein was immunoprecipitated from the nuclear extract of monocyte–macrophage (MonoMac6) cells treated with CSE (0.1, 0.5, and 1.0%) for 4 hours. The levels of SIRT1 adducts with 4-hydroxy-2-nonenol (4-HNE) and nitration of tyrosine residues on SIRT1 were analyzed by immunoblotting with anti–4-HNE and anti–3-nitrotyrosine (3-NT) antibodies, respectively. Equal amount of immunoprecipitated SIRT1 protein (100 μg) was used for Western blotting. Relative intensity of 4-HNE/SIRT1 (B) and 3-NT/SIRT1 (C) represents the increased post-translational modifications of SIRT1 protein in response to CSE treatment. Results are means ± SEM of three separate experiments (n = 3). Significant differences are shown as compared to controls: *P < 0.05, **P < 0.01, and ***P < 0.001.