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. Author manuscript; available in PMC: 2008 Apr 14.
Published in final edited form as: Neuroscience. 2007 Dec 8;151(4):969–982. doi: 10.1016/j.neuroscience.2007.11.045

Figure 6.

Figure 6

Regional distribution of R7BP protein expression in adult mouse brain. Free-floating frozen sections of brain were probed as indicated with unblocked or blocked anti-R7BP antibodies (chicken), followed by anti-chicken-HRP conjugates and visualization by DAB staining. A) Specificity of R7BP antibody staining. Sagittal sections of brains from adult wild type mice that express R7BP were compared with sections from brains of adult Gβ5-/- mice or E19.5 wild type mice, which do not express R7BP. B) Anti-R7BP antibody staining of sagittal sections and a series of coronal sections (0.5 mm to 3.28 mm from the bregma suture). Abbreviations: ec, cerebellum; CPu, caudate-putamen; cx, cortex; hip, hippocampus; th, thalamus; LGP, lateral globus pallidus; sth, subthalamic nucleus; hth, hypothalamus; PAG, periaqueductal grey; MG, medial geniculate nucleus; SN, substantia nigra; Ip, interpeduncular nucleus. Results shown are representative of more than five independent experiments in which sections of brains from 3 animals were used.