Table 1.

Expression pattern of R7BP in adult mouse brain detected by DAB staining

Neocortex
Layer I/ II/III	+++
Layer IV	++
Layer V	++
Layer VI	+++
Basal ganglia
Caudate-putamen	+++
Globus pallidus	+
Subthalamic nucleus	+++
Ventral striatum	+
Brainstem
Periaqueductal grey	+
substantia nigra pars reticulate	++
substantia nigra pars compacta	-
Ventral tegmental area	-
Hippocampus
CA1/ CA2	+++
CA3	+++
DG	+++
Thalamus
Ventrobasal nuclei	+++
Geniculate bodies	+++
Anterior nuclei	+
Midline nuclei	+
Hypothalamus	+
Cerebellum
Purkinje cells	++++
Granular cell layer	+++
Molecular cell layer	++
Olfactory bulb
Olfactory nerve layer	+++
Glomerular layer	++
Mitral cell layer	++
Granular cell layer	++

Semi-quantitive evaluation of R7BP staining was done by visual inspecting serial coronal and sagittal sections probed with anti-R7BP antibodies, followed by anti-chicken-HRP conjugates and visualized by DAB staining. Two investigators independently scored the same immunohistochemical sections. Staining intensities relative to the highest level observed (Purkinje cell layer) are indicated as follows: very dense (equivalent to that in the Purkinje cell layer), ++++; dense, +++; moderately dense, ++; light (visible above background), +; not detectable (indistinguishable from nonspecific background), -.