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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Dev Cell. 2008 Feb;14(2):275–286. doi: 10.1016/j.devcel.2007.12.005

Figure 2. SOX100B-expressing cells give rise to testis PCs.

Figure 2

St. 17 gonads and adult testes immunostained as indicated in the figure. Anterior is to the left in each panel. (A,A’) Kr-GAL4, UAS-GFP adult testis examined by either light microscopy (A) or immunofluorescence (B). Inset: Slice of three-dimensional reconstruction of testis. Note that GFP expression is coincident with the yellow pigmentation of the testis and is only in the outer layer of cells. (B,C) The B-57 enhancer trap labels PCs in the adult testis (B) and st. 17 embryonic male gonad (C), and co-localizes with SOX100B (arrowheads). (D,E) Adult testes showing SOX100B-positive PCs in both wild-type (D) and AbdB[HCJ199] mutants (E). The AbdB[HCJ199] mutant testes do not have the normal, elongated appearance of wild-type testes, likely due to defects in interaction with the genital disc. (F,G) St. 17 embryonic male gonads immunostained to reveal msSGPs (co-expressing ABD-B and SOX100B) and PCs (SOX100B only, arrowheads). In addition, a few posterior SGPs also co-express ABD-B and SOX100B (DeFalco et al., 2003). Note that PCs are observed in wild-type (F) but not Wnt2 mutants (G).