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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Dev Cell. 2008 Feb;14(2):275–286. doi: 10.1016/j.devcel.2007.12.005

Figure 6. Wnt2 acts non-autonomously to regulate sex-specific PC precursor specification.

Figure 6

(A-C) St. 17 embryos labeled by fluorescent in situ hybridization for Wnt2 mRNA (red) and anti-VASA immunostaining (green). Anterior is to the left in each panel. (A’-C’) Wnt2 mRNA channel alone. (A,B) Wild type embryos. Wnt2 expression is robust in male embryonic gonads at st. 17 (B,B’) but is not detectable above background in females (A,A’) at this stage. (C) dsx1/dsx23 mutant gonad. Wnt2 expression is now observed in 100% of embryos. (D-G) St. 17 embryos immunostained as indicated in the figure. (D) Female embryo exhibiting SOX100B-positive PC precursors when Wnt2 is ectopically expressed in the germ cells (UAS-Wnt2, nos-GAL4). (E-G) Embryos expressing prd-GAL4 and UAS-GFP either alone (E), or in combination with UAS-dTCFΔN (F) or UAS-sggact (G). Note that some GFP-positive cells become SOX100B-positive PC precursors when expressing GFP alone (E, arrowheads), but not when expressing UAS-dTCFΔN (F) or UAS-sggact (G).