FIG. 4.

Coomassie blue-stained SDS-polyacrylamide gel electrophoresis gel showing purification of the rMsp22 (22 kDa), rMsp75 (75 kDa), and rMsp78 (78 kDa) proteins. Lanes 1, standard; lanes 2, unbound supernatant after protein was bound to cobalt resin; lanes 3, cobalt resin after elution of protein with imidazole, showing some protein that remained bound to the resin and did not elute; lanes 4, eluted protein after diafiltration into buffer Z1. The positions of molecular mass standards (in kilodaltons) are indicated on the left.