TABLE 3.
ELISA cutoff value determination for the paired serum and sputum samples
| Ig | % Change
|
|||||
|---|---|---|---|---|---|---|
| rMsp22
|
rMsp75
|
rMsp78
|
||||
| Mean ± SD | 99% CIa | Mean ± SD | 99% CI | Mean ± SD | 99% CI | |
| Serum IgG | −2.5 ± 8.1 | 18.4 | −40.0 ± 25.5 | 25.8 | −21.1 ± 15.1 | 17.7 |
| Sputum IgA | 61.9 ± 137.4 | 416.4 | 0 ± 0b | 0 | −10.3 ± 31.0 | 69.6 |
a
99% CI, upper limit of the 99% confidence interval for the control samples. Any percent change in the OD450 greater than the upper limit of the 99% confidence interval between the preacquisition and postclearance values for a protein was considered a significant change.
b
An OD450 less than 0.1 was considered not to represent a significant level of antibody, and a value of zero was assigned.