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. 2003 Nov;77(21):11651–11660. doi: 10.1128/JVI.77.21.11651-11660.2003

FIG. 3.

FIG. 3.

G2A-Gag-YFP rescue by MLV Gag-Pol. (A) Supernatants of 293T cells transfected with the indicated constructs were fixed on glass slides, and the fluorescent particles were visualized by confocal microscopy. wt, wild type. (B) Supernatants of 293T cells cotransfected with G2A-Gag-YFP and MLV Gag-Pol (WT Gag Pol) were analyzed as described in the legend to Fig. 1C. (C) Supernatants of 293T-MoAmpho (lanes 1), 293T-MLV-A (lanes 2), and 293T (lanes 3) cells, either nontransfected (MOCK) or transfected with Gag-YFP (1:10 diluted) or G2A-Gag-YFP, were harvested, concentrated, and analyzed by immunoblotting with anti-CA or anti-GFP antibodies. A short exposure (SE, 1 min) for the top of the blot and a long exposure (LE, 15 min) for the bottom of the blot are shown. Numbers at left in panels B and C indicate kilodaltons.