FIG. 6.
FNd produced by leishmanolysin enhances the intracellular growth of L. amazonensis by diminishing the production of ROI. Mφs (5 × 105) bound to coverslips were incubated with 10 μg of FNi (gray bars), FNd (white bars), or buffer alone (black bars) for 30 min prior to the addition of late-stationary-phase promastigotes. (A) Growth of intracellular amastigotes within Mφs. (B) Measurement of ROI produced within infected Mφs. Means ± standard deviations are shown. The asterisks over the horizontal lines denote statistically significant differences (P ≤ 0.05) between the column under the asterisk and the spanned value(s).